Retention Behavior of Polyethylene Glycol and Its Influence on Protein Elution on Hydrophobic Interaction Chromatography Media
نویسندگان
چکیده
منابع مشابه
A comprehensive study to protein retention in hydrophobic interaction chromatography.
The effect of different kosmotropic/chaotropic salt systems on retention characteristics of intact proteins has been examined in hydrophobic interaction chromatography (HIC). The performance was assessed using different column chemistries, i.e., polyalkylamide, alkylamine incorporating hydrophobic moieties, and a butyl chemistry. Selectivity in HIC is mainly governed by the salt concentration a...
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ژورنال
عنوان ژورنال: Chromatographia
سال: 2018
ISSN: 0009-5893,1612-1112
DOI: 10.1007/s10337-018-3635-9